Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein     

罗本燕  徐增斌  陈智  陈峰  唐敏 《浙江大学学报(A卷英文版)》2004,5(8):989-994

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.  相似文献   

Clinical analysis of minimally invasive McKeown esophagectomy in a single center by a single medical group     

Zi-yi Zhu  Xu Yong  Rao-jun Luo  Yun-zhen Wang 《Journal of Zhejiang University. Science. B》2018,19(9):718-725

Objective: McKeown esophagectomy followed by cervical and abdominal procedures has been commonly used for invasive esophageal carcinoma. This minimally-invasive operative procedure in the lateral prone position has been considered to be the most appropriate method. We describe our experiences in minimally invasive McKeown esophagectomy (MIME) for esophageal cancer. Methods: Between March 2016 and February 2018, a total of 82 patients underwent MIME by a single group in our department (a single center). All procedure, operation, oncology, and complication data were reviewed. Results: All MIME procedures were completed successfully, with no conversions to open surgery. The median operative time was 260 min, and median blood loss was 100 ml. The average number of total harvested lymph nodes was 20.1 in the chest and 13.5 in the abdomen. There were no deaths within 30 postoperative days. Twenty cases (24.4%) developed postoperative complications, including anastomotic leak in 4 (4.9%), single lateral recurrent nerve palsy in 4 (4.9%), bilateral recurrent nerve palsy in 1 (1.2%), pulmonary problems in 3 (3.7%), chyle leak in 1 (1.2%), and other complications in 7 (including pleural effusions in 4, incomplete ileus in 2, and neck incision infection in 1; 8.54%). Average postoperative hospitalization time was 12 d. Blood loss, operation time, morbidity rate, and the number of harvested lymph nodes were analyzed by evaluating learning curves in different periods. Significant differences were found in operative time (P=0.006), postoperative hospitalization days (P=0.015), total harvested lymph nodes (P=0.003), harvested thoracic lymph nodes (P=0.006), and harvested abdominal lymph nodes (P=0.022) among different periods. Conclusions: Surgical outcomes following MIME for esophageal cancer are safe and acceptable. The MIME procedure for stages I and II could be performed proficiently and reached an experience plateau after approximately 25 cases.  相似文献   

开放、多元、综合的艺术教育——美国艺术教育考察报告   总被引：5，自引：0，他引：5  

罗海鸥 《教育研究》2003,(1)

本文通过对美国艺术教育的实地考察和分析 ,指出美国艺术教育是在人文科技视野下实施的开放、多元、综合的艺术教育 ,具有如下几个特点 :从教育内容看 ,是跨学科、多层面、综合的 ;从教学方法看 ,是探究性和多元化的 ;从教育途径看 ,是学校、社会和家庭积极合作、互动的 ;艺术教师队伍结构灵活、专兼并举、素质较高。作者还就改革和加强我国艺术教育提出了若干思考  相似文献   

关于我国换股合并会计处理方法的探讨     

罗慧 《广西大学梧州分校学报》2005,15(4):7-9

尽管权益结合法存在许多缺陷，但由于购买法目前在我国尚不具备现实运用环境，且为了促进我国企业合并的发展，现阶段应允许我国进行换股合并的食业采采权豁结合法。  相似文献   

构建现代教育服务体系的理论与实践   总被引：1，自引：0，他引：1  

靳雁涛  骆东升 《四川教育学院学报》2007,23(12):22-24

大连教育学院为了很好地服务于本市新时期的初、中等教育,坚持科学发展观,积极构建现代教育服务体系:以"原野行动"服务于新农村建设的教师培训;以"大篷车计划"构建服务于教师专业发展的网络平台;以"空中课堂"服务于农村小学的英语教学;以"村小校长培训"服务于推进区域教育均衡发展的校长培训末梢工程;以"名师名校长培训"服务于全面实施素质教育的校长、教师培训;以"二二三四"工作服务于大连市的初、中等教育与教学。  相似文献   

2015年高职高专全国大学英语竞赛测试分析与探讨     

罗红霞 《佳木斯教育学院学报》2016,(4):370

本文以2015年高职高专全国大学英语竞赛测试真题为例,对试题的结构、考察内容、测试题型、题量、分值比例进行归类量化分析,发现该竞赛试题的题量与难度呈逐年上升的趋势,测试内容愈加广泛,题型多样化的特点愈加明显;从而探讨该竞赛的测试特点与发展趋势,充分发挥大学英语竞赛对英语教学实践的促进作用。  相似文献   

利用语音识别引擎实现电脑英语语音测试的探索     

罗军平 《中国教育技术装备》2005,(1):19-20

介绍了作研发的一套英语语音测试系统软件，能利用计算机判断识别学生的语言，可代替教师进行英语语音测试的繁重工作。  相似文献   

Kinetics study on biomass pyrolysis for fuel gas production     

陈冠益  方梦祥  ANDRIES J  骆仲泱  SPLIETHOFF H  岑可法 《浙江大学学报(A卷英文版)》2003,4(4):441-447

Kinetic knowledge is of great importance in achieving good control of the pyrolysis and gasification process and optimising system design. An overall kinetic pyrolysis scheme is therefore addressed here. The kinetic modelling incorporates the following basic steps: the degradation of the virgin biomass materials into primary products (tar, gas and semi-char), the decomposition of primary tar into secondary products and the continuous interaction between primary gas and char. The last step is disregarded completely by models in the literature. Analysis and comparison of predicted results from different kinetic schemes and experimental data on our fixed bed pyrolyser yielded very positive evidence to support our kinetic scheme. Project (No. 2001CB409600) Supported by the National Key Basic Research Program (973) of China  相似文献   

前景化理论与英语新闻报道词汇的文体特征分析   总被引：1，自引：0，他引：1  

罗晓君 《四川教育学院学报》2001,17(3):54-55,58

系统功能语言学家韩礼德摒弃了偏离学的观点，把前景化定义有“有动因的突出”，主张从功能的角度研究文体，即每个次上的有文化价值的突出特征都要与产生本语篇的语境相关，都应是由上层所“促动的”，韩氏的理论是本文对英语新闻报道的文体特征分析的理论基础，英语新闻报道的词汇的文体特征是由其情景因素决定的，它的突出特征是受其语义特色促动的。  相似文献   

Reform of Mathematics Curriculum and Open-ended Problem Solving     

王仕政  罗新兵 《中学数学教学参考》2006,(1):116-116

The basic assumption of reform mathematics is that ＂no one can teach mathematics＂. Therefore, the important teachers＇ role is to stimulate students to learn mathematics and support their development. Open-ended problem solving is an instructional strategy that creates interest and stimulates creative mathematical activity in the classroom through students＇ collaborative work. Lessons using openended problem solving problem solving activities result. emphasize the process of rather than focusing on the  相似文献